Studying the hotbed of horizontal gene transfers

For over 200,000 years, humans and their gut microbiomes have coevolved into some of the most complex collections of living organisms on the planet. But as human lifestyles vary from the urban to rural, so do the bacterial diversities of gut microbiomes.

Studying the hotbed of horizontal gene transfers
A woman in Ghana carries a basket of fish. Local diets and lifestyles have created a remarkable
diversity in the human microbiome worldwide [Credit: Mathieu Groussin]

That’s why a nonprofit global collaboration of more than 70 scientists has been working for the past two years to interrogate and preserve that diversity of gut microbiomes from populations around the world, racing against the proliferation of antibiotic overuse and Western-style diets, which are known to contribute to a decrease in gut bacterial diversity. The Global Microbiome Conservancy, launched in 2016, already has some surprising insights that further shed light on the black box of gut bacterial diversity and human digestion.

The MIT-based scientists who founded the conservancy come from a disparate set of disciplines: biological engineering, evolutionary biology, organic geochemistry, and biomedical research. Such collaborations may allow for interdisciplinary innovation that stands to improve the understanding of the science within our guts, says Mathilde Poyet, a postdoc in the Department of Biological Engineering’s Alm Lab. Poyet believes the interdisciplinary nature of the collaboration promotes the conservancy’s goals, which she says are to “preserve the full biodiversity of the human gut microbiome before it is lost, and to advance our understanding of the origins and functions of the human gut microbiomes,” within a “robust research ecosystem.”

Poyet and fellow MIT-based postdocs Mathieu Groussin, who also works in the Alm Lab, and Ainara Sistiaga, a Marie Curie Postdoctoral Fellow in the Summons Lab within the Department of Earth, Atmospheric and Planetary Sciences (EAPS), presented the preliminary results of their work at the first MIT Microbiome Club meeting of the 2018-19 academic year. At the meeting, Poyet, Groussin, and Sistiaga spoke about their research progress as well as what’s surprised them most so far.

In addition to biobanking and characterizing bacterial isolates collected from fecal samples from around the world, Poyet focuses on the horizontal exchange of genetic materials between living organisms, known as horizontal gene transfer (HGT). Unlike vertical transfers, horizontal gene transfers are acquisitions of genetic material from non-parental lineages.

“Transfers of genes are known to be crucial for bacterial evolution and the human gut microbiome is an important hotbed for horizontal gene transfers,” Poyet says. “But all previous studies fell short at characterizing the timescale of these HGT events.”

With the collection of bacterial isolates and genomes she built, Poyet is now able to identify evidence of very recent horizontal gene transfers, supporting the notion that these transfers frequently occur during the human lifetime. The effects of highly active horizontal gene transfers mean that gut bacteria could experience a sort of turnover of genetic material over time, which the conservancy hopes to study further as they collect more data.

Studying the hotbed of horizontal gene transfers
Harvesters gather crops on a farm in Rwanda. The Global Microbiome Conservancy’s current studies focus on samples
collected from 19 distinct populations sampled from sub-Saharan countries, Arctic regions, and North America
[Credit: Christopher Corzett]

From additional preliminary analysis, Poyet and Groussin have found that in industrialized populations, which tend to have less gut bacterial diversity, meaning the rate of gene exchange through horizontal gene transfer is greater. “As HGT is a mechanism frequently used by bacteria to rapidly adapt to changing environments, this result suggests that an industrialized lifestyle imposes a stronger selective pressure on the gut microbiome of individual people, possibly as a result of the high consumption of antibiotics,” Poyet says.

Ainara Sistiaga has been zeroing in on a dietary element that could also have implications for gastrointestinal bacteria and human health: lipids. Lipids, the hydrophobic biomolecules often referred to as fats, are unexplored in the gut microbiome. Sistiaga aims to change that.

With fecal samples collected through the conservancy, Sistiaga performs lipid analysis—a series of chemistry-rooted procedures that help researchers characterize which lipids are present as well as their abundances.

One lipid of particular interest is cholesterol, a molecule widely implicated in heart disease and stroke. As meat-heavy diets increase in popularity around the world, global cholesterol intake is set to rise as well. Theoretically, in most humans, some of the cholesterol that reaches the gut should undergo a bacterial conversion into a byproduct that the body cannot reabsorb and would be excreted. But Sistiaga finds that all gut microbiomes execute metabolic functions differently. “What we have observed is that most of the participants in rural populations are performing this conversion much more efficiently than Western donors,” Sistiaga says. So, in order to describe and understand how such processes work within the gut, Sistiaga hopes to use methods informed from organic geochemistry to characterize the fate of cholesterol in humans from around the world.

After the presentation, the MIT researchers fielded questions from the audience. “People were interested in all aspects of the presentation—traveling, how to get involved, the science, the preliminary results, the potential outcomes of this project,” Sistiaga says. “Even after the Q&A, a lot of people approached us for more specific questions.”

As part of its efforts, the Global Microbiome Conservancy establishes long-term collaborations with local populations and researchers for sampling and analysis—and the participants themselves maintain ownership of their samples. Within the next two years, the conservancy is set to increase its global coverage. The current microbiome studies conducted focus on samples collected from 19 distinct populations sampled worldwide from Arctic regions, sub-Saharan countries and North America, but they’ll soon be complemented with samples from Malaysia and Peru. Poyet adds, “We anticipate [samples from Asia and South America] will yield novel and unique microbial communities that must be preserved.”

“It’s been exciting to unveil the microbiome of these underrepresented communities, and I think we are just seeing the tip of the iceberg,” Sistiaga says. “Everything we’ve observed so far confirms how important and truly urgent this work is.”

Author: Fatima Husain | Source: Massachusetts Institute of Technology [October 22, 2018]



Block Patterns At the earliest stage of our lives, genes…

Block Patterns

At the earliest stage of our lives, genes switch on and off inside stem cells, guiding them towards different destinies in future tissues and organs. Here a technique called CRISPR interference blocks specific genes inside groups of human pluripotent stem cells, mimicking some of the early decisions made inside embryos. A high-powered microscope watches how artificially-coloured cell clumps develop differently over one, three and five days (across each row). The red-coloured cells have a switched-off ROCK1 gene (middle) and move towards the edge, while blocking CDH1 (bottom, blue) directs cells into little islands, compared to the white cells with normal genes (top). This technique may be used to study the effects of other developmental genes, creating realistic models for delicate embryonic stages. These may reveal insights into miscarriage or perhaps developmental disorders, as well as morphogenesis – the unseen patterns that shape early life.

Written by John Ankers

You can also follow BPoD on Instagram, Twitter and Facebook

Archive link

HiPOD (22 October 2018): Flow Lobes in a Northern Plains Crater…

HiPOD (22 October 2018): Flow Lobes in a Northern Plains Crater

   – This is a re-image of an area to help us check for potential tectonic activity. Mars does not have tectonic plates, so we are referring to the effects of gravity. (321 km above the surface. Black and white is less than 5 km across; enhanced color is less than 1 km.)

NASA/JPL/University of Arizona